Spindle cell lesions
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Spindle cell lesions are seen frequent enough that one ought to have a solid approach to 'em.
General DDx
Spindle cells should make think:
- Nerve.
- Muscle.
- Smooth.
- Skeletal.
- Other.
IHC
Basic panel
Dixon panel:[1]
- CD34.
- Desmin.
- SMA.
- S100.
- AE1/AE3 (pankeratin).
Big panels
A MSH MFH panel
- CD34 - GIST, angiosarcoma, solitary fibrous tumour/hemangiopericytoma, Kaposi sarcoma, DFSP.
- S100 - neural marker, sensitive for malignant melanoma, Langerhans cells, clear cell sarcoma.
- Desmin - smooth muscle.
- MIB1 - proliferation.
- CD99 - immature T cells (thymus), small round cell tumours, synovial sarcoma, merkel cell carcinoma, osteosarcoma.[2]
- Considered "slutty", i.e. non-specific, by Dr. Fletcher; useful if membranous stain pattern = Ewing sarcoma/PNET.
- BCL2 - synovial sarcoma, small cell lymphomas, spindle cell lipoma.[3][4]
- PGP 9.5.
- SMMS - smooth muscle.
- Caldesmon - muscle.
- PDGFR - GIST
- DOG-1 - GIST (membranous, cytoplasmic),[5] normal pancreas (islets),[5] associated with BRCA1.
Others:
- Beta-catenin - a small subset of soft tissue lesions:
- Desmoid-type fibromatosis (~70%), solitary fibrous tumour & endometrial stromal sarcoma (~40%), synovial sarcoma (~30%).[6]
DJH panels
MFH
- Vimentin.
- Keratin 5D3 - carcinomas.
- Keratin LP34 - carcinomas.
- EMA.
- CD99.
- MSA.
- SMA.
- Caldesmon.
- CD34.
- S-100 - neural tumours.
- HMB45 - melanoma
- Mart-1 - melanoma.
- Tyrosinase.
- MYF-4 - RMS.
- Alpha-1-antichymotrypsin.
SRCT
Same as MFH panel plus the following:
- CD45.
- CD30.
- CD3.
- CD20.
- Desmin.
- CD31 - angiosarcoma.
- Factor VIII - angiosarcoma.
See also
References
- ↑ BD. 1 April 2011.
- ↑ Tadrous, Paul.J. Diagnostic Criteria Handbook in Histopathology: A Surgical Pathology Vade Mecum (1st ed.). Wiley. pp. 108. ISBN 978-0470519035.
- ↑ Tadrous, Paul.J. Diagnostic Criteria Handbook in Histopathology: A Surgical Pathology Vade Mecum (1st ed.). Wiley. pp. 107. ISBN 978-0470519035.
- ↑ URL: http://ajp.amjpathol.org/cgi/content/full/160/3/759. Accessed on: 3 August 2010.
- ↑ 5.0 5.1 Ardeleanu C, Arsene D, Hinescu M, et al. (October 2009). "Pancreatic expression of DOG1: a novel gastrointestinal stromal tumor (GIST) biomarker". Appl. Immunohistochem. Mol. Morphol. 17 (5): 413–8. doi:10.1097/PAI.0b013e31819e4dc5. PMID 19417627.
- ↑ Ng TL, Gown AM, Barry TS, et al. (January 2005). "Nuclear beta-catenin in mesenchymal tumors". Mod. Pathol. 18 (1): 68–74. doi:10.1038/modpathol.3800272. PMID 15375433.