Difference between revisions of "Tissue fixation"
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'''Tissue fixation''', usually just '''fixation''', is an important part of tissue preparation for histologic examination. It is typically done with formulin. | '''Tissue fixation''', usually just '''fixation''', is an important part of tissue preparation for histologic examination. It is typically done with formulin. | ||
==Fixing marking dye== | |||
To fix marking dye: | To fix marking dye: | ||
*Formal-acetic alcohol (FAA):<ref>URL: [http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html]. Accessed on: 5 January 2011.</ref> | *Formal-acetic alcohol (FAA):<ref>URL: [http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html]. Accessed on: 5 January 2011.</ref> | ||
*Bouin's solution. | *Bouin's solution. | ||
== | ==Tissue fixation== | ||
A list of fixatives:<ref name=pmid18251585>{{Cite journal | last1 = Hunt | first1 = JL. | title = Molecular pathology in anatomic pathology practice: a review of basic principles. | journal = Arch Pathol Lab Med | volume = 132 | issue = 2 | pages = 248-60 | month = Feb | year = 2008 | doi = 10.1043/1543-2165(2008)132[248:MPIAPP]2.0.CO;2 | PMID = 18251585 | url = http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165%282008%29132%5B248%3AMPIAPP%5D2.0.CO%3B2 }} | |||
</ref> | |||
{| class="wikitable sortable" style="margin-left:auto;margin-right:auto" | |||
! Fixative | |||
! Comment | |||
|- | |||
| Formalin, neutral buffered | |||
| standard fixative | |||
|- | |||
| Formalin, unbuffered | |||
| ??? | |||
|- | |||
| Glutaraldehyde | |||
| [[electronmicroscopy]] ??? | |||
|- | |||
| Ethanol | |||
| [[cytopathology]] ??? | |||
|- | |||
| Carnoy | |||
| ??? | |||
|- | |||
| Bouin | |||
| toxic ??? | |||
|} | |||
=Tissue fixation= | |||
==Formalin== | |||
*Formaldehyde. | *Formaldehyde. | ||
=Dye fixation= | |||
==Formal-acetic-alcohol== | ==Formal-acetic-alcohol== | ||
General: | General: | ||
| Line 21: | Line 47: | ||
*Methanol. | *Methanol. | ||
=See also= | |||
*[[Basics]]. | *[[Basics]]. | ||
=References= | |||
{{Reflist| | {{Reflist|2}} | ||
=External links= | |||
*[http://www.wisegeek.com/what-is-formalin.htm What is formulin (wisegeek.com)]. | *[http://www.wisegeek.com/what-is-formalin.htm What is formulin (wisegeek.com)]. | ||
*[http://en.wikipedia.org/wiki/Fixation_%28histology%29 Fixation (WP)]. | *[http://en.wikipedia.org/wiki/Fixation_%28histology%29 Fixation (WP)]. | ||
[[Category:Basics]] | [[Category:Basics]] | ||
Revision as of 16:00, 9 May 2011
Tissue fixation, usually just fixation, is an important part of tissue preparation for histologic examination. It is typically done with formulin.
Fixing marking dye
To fix marking dye:
- Formal-acetic alcohol (FAA):[1]
- Bouin's solution.
Tissue fixation
A list of fixatives:[2]
| Fixative | Comment |
|---|---|
| Formalin, neutral buffered | standard fixative |
| Formalin, unbuffered | ??? |
| Glutaraldehyde | electronmicroscopy ??? |
| Ethanol | cytopathology ??? |
| Carnoy | ??? |
| Bouin | toxic ??? |
Tissue fixation
Formalin
- Formaldehyde.
Dye fixation
Formal-acetic-alcohol
General:
- Different recipes exist.
One recipe:[3]
- Ethanol.
- Acetic acid.
- Formaldehyde.
- Methanol.
See also
References
- ↑ URL: http://www.brunelmicroscopes.co.uk/acatalog/Solvents___Reagents.html. Accessed on: 5 January 2011.
- ↑ Hunt, JL. (Feb 2008). "Molecular pathology in anatomic pathology practice: a review of basic principles.". Arch Pathol Lab Med 132 (2): 248-60. doi:10.1043/1543-2165(2008)132[248:MPIAPP]2.0.CO;2. PMID 18251585. http://www.archivesofpathology.org/doi/pdf/10.1043/1543-2165%282008%29132%5B248%3AMPIAPP%5D2.0.CO%3B2.
- ↑ URL: https://fscimage.fishersci.com/msds/45357.htm. Accessed on: 5 January 2011.