Difference between revisions of "Bone"

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=Normal bone=
=Normal bone=
==Bone==
==Bone anatomy==
*Normal bone has osteocytes.
*Epiphysis = rounded end of the bone.
**If the osteocytes are missing... the bone is dead.
*Metaphysis = between epiphysis and diaphysis; contains ''epiphyseal plate'' - site of growth in childhood.
*Diaphysis = shaft, mid-portion, contains [[bone marrow]].


===Matrix===
===Image===
<gallery>
Image:EpiMetaDiaphyse.jpg | Bone. (WC)
</gallery>
 
==Bone histology==
Two types (based on arrangement of collagen):
Two types (based on arrangement of collagen):
#Woven.  
# Woven bone.
#*Always abnormal in adults.
# Lamellar bone.
#*Collagen arranged haphazardly - mechanically weak.
 
#Lamellar.
===Woven bone===
#*Collagen organized in layers (lamellae).
*Always abnormal in adults.
*Collagen arranged haphazardly - mechanically weak.  


Images:
Images:
Line 20: Line 27:
*[http://www.brown.edu/Courses/Digital_Path/systemic_path/bone/paget.html Woven bone (brown.edu)].
*[http://www.brown.edu/Courses/Digital_Path/systemic_path/bone/paget.html Woven bone (brown.edu)].


===Cells===
===Lamellar bone===
[[File:Lamella bone H&E and under polarised light.gif|thumb|Animated GIF showing a photomicrograph of lamellar bone. The layers are accentuated under polarised light.]]
*Collagen organized in layers (lamellae).
 
===Woven versus lamellar===
*Easiest way to differentiate: polarize; lamellar bone has well-defined layers.
 
==Bone cells==
*Osteocytes.
*Osteocytes.
**Sit in lacunae.
**Sit in lacunae.
Line 34: Line 48:
==Bone marrow==
==Bone marrow==
{{Main|Haematopoiesis}}
{{Main|Haematopoiesis}}
*Fat content (%) ~= age (in years)<ref>IAV. 26 Feb 2009.</ref>
===General===
**e.g. 60 year old will have 60% fatty replacement.
*One of two ''[[primary lymphoid organs]]'' - the other one is the [[thymus]].<ref>URL: [http://www.life.umd.edu/classroom/bsci423/song/Lab1.html http://www.life.umd.edu/classroom/bsci423/song/Lab1.html]. Accessed on: 28 March 2012.</ref>


*Should see three cell lines.
Bone marrow biopsy adequacy:<ref name=pmid16477878>{{Cite journal  | last1 = Rudzki | first1 = Z. | last2 = Partyła | first2 = T. | last3 = Okoń | first3 = K. | last4 = Stachura | first4 = J. | title = Adequacy of trephine bone marrow biopsies: the doctor and the patient make a difference. | journal = Pol J Pathol | volume = 56 | issue = 4 | pages = 187-95 | month =  | year = 2005 | doi =  | PMID = 16477878 }}</ref>
**The cell lines:<ref>[http://emedicine.medscape.com/article/199003-overview http://emedicine.medscape.com/article/199003-overview]</ref>
*10 intertrabecular spaces or 10 mm.
***Erythroid (red cells),
***Myeloid (white blood cells),
***Megakaryocytic (platelets).


Note: Lymphocytes are considered separately and typically spared in bone marrow failure.<ref>[http://emedicine.medscape.com/article/199003-overview http://emedicine.medscape.com/article/199003-overview]</ref>
===Microscopic===
One should see three cell lines:<ref>URL: [http://emedicine.medscape.com/article/199003-overview http://emedicine.medscape.com/article/199003-overview]. Accessed on: 28 March 2012.</ref>
#Erythroid (red cells).
#Myeloid (white blood cells).
#Megakaryocytic (platelets).


Identifying the lines:<ref>[http://upload.wikimedia.org/wikipedia/commons/6/69/Hematopoiesis_%28human%29_diagram.png http://upload.wikimedia.org/wikipedia/commons/6/69/Hematopoiesis_%28human%29_diagram.png]</ref>
Adipose tissue:
*Fat content (%) ~= age in years.<ref>IAV. 26 Feb 2009.</ref>
**e.g. 60 year old will have 60% fatty replacement.
 
Identifying the cell lines:<ref>[http://upload.wikimedia.org/wikipedia/commons/6/69/Hematopoiesis_%28human%29_diagram.png http://upload.wikimedia.org/wikipedia/commons/6/69/Hematopoiesis_%28human%29_diagram.png]</ref>
#Megakaryocytes:
#Megakaryocytes:
#*Big cells ~ 3x the size of a RBC.
#*Big cells ~ 3x the size of a [[RBC]].
#Normoblasts (RBC precursors):
#[[Normoblast]]s (RBC precursors):
#*Hyperchromatic, i.e. blue, nucleus.
#*Hyperchromatic, i.e. blue, nucleus.
#Myeloid line:
#Myeloid line:
Line 54: Line 73:
#*Reniform nucleus, i.e. kidney bean shaped nucleus.
#*Reniform nucleus, i.e. kidney bean shaped nucleus.


Images:
Note:  
*[http://commons.wikimedia.org/wiki/File:Bone_marrow_WBC.JPG Myeloid line (WC)].
*Lymphocytes are considered separately and typically spared in bone marrow failure.<ref>[http://emedicine.medscape.com/article/199003-overview http://emedicine.medscape.com/article/199003-overview]</ref>
 
====Organization====
====Organization====
*Mature hematopoeitic cells at the centre (distant from bone).
*Mature hematopoeitic cells at the centre (distant from bone).
*Immature hematopoeitic cells adjacent to the bone.
*Immature hematopoeitic cells adjacent to the bone.
Note:
*Immediately adjacent to the bone (paratrabecular)... is where [[follicular lymphoma]] is classically found.<ref name=pmid17284114>{{Cite journal  | last1 = Iancu | first1 = D. | last2 = Hao | first2 = S. | last3 = Lin | first3 = P. | last4 = Anderson | first4 = SK. | last5 = Jorgensen | first5 = JL. | last6 = McLaughlin | first6 = P. | last7 = Medeiros | first7 = LJ. | title = Follicular lymphoma in staging bone marrow specimens: correlation of histologic findings with the results of flow cytometry immunophenotypic analysis. | journal = Arch Pathol Lab Med | volume = 131 | issue = 2 | pages = 282-7 | month = Feb | year = 2007 | doi = 10.1043/1543-2165(2007)131[282:FLISBM]2.0.CO;2 | PMID = 17284114 }}</ref>
**Image: [http://www.flickr.com/photos/euthman/5691578596/in/set-72057594114099781 Paratrabecular follicular lymphoma (flickr.com)].
===Images===
<gallery>
Image:Bone_marrow_WBC.JPG| Myeloid line. (WC)
</gallery>
===Sign out===
====Essentially normal====
<pre>
Bone Marrow, Posterior Superior Iliac Spine, Biopsy:
- Bone marrow with trilineage hematopoiesis.
- Cellularity within normal limits for age.
- Case will be sent to hematology for consultation and correlation
  with other testing.
</pre>
====Inadequate====
<pre>
Right Posterior Superior Iliac Spine, Bone Marrow Biopsy:
- Predominantly benign dense fibrous tissue with a small
  amount of laminar bone, and minute fragment of marrow (<2 mm).
- Case will be sent to hematopathology for further assessment.
</pre>


=Benign variants=
=Benign variants=
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=Infections=
=Infections=
==Osteomyelitis==
==Acute osteomyelitis==
General
===General===
Pathophysiology/entry:
*Hematogenous - often in children.
*Hematogenous - often in children.
*Direct entry (skin defect) - adults with diabetes.
*Direct entry (skin defect) - adults with diabetes.


===Microscopic===
===Microscopic===
Features:
*[[PMN]]s.
*[[PMN]]s.
*Micro-organisms - esp. cocci.
Image:
*[http://osteomyelitis.stanford.edu/pages/histo_002.html Acute osteomyeelitis (stanford.edu)].
===Stains===
*[[Gram stain]].


==Chronic osteomyelitis==
==Chronic osteomyelitis==
*Plasma cells.
===General===
Most common organism:
*''Staphylococcus aureus''.<ref name=pmid22046943>{{Cite journal  | last1 = Hatzenbuehler | first1 = J. | last2 = Pulling | first2 = TJ. | title = Diagnosis and management of osteomyelitis. | journal = Am Fam Physician | volume = 84 | issue = 9 | pages = 1027-33 | month = Nov | year = 2011 | doi =  | PMID = 22046943 | URL = http://www.aafp.org/link_out?pmid=22046943 }}</ref>
 
Risk factors:<ref name=pmid22046943/>
*[[Diabetes mellitus]].
*Peripheral vascular disease.
 
===Microscopic===
Features:
*Plasma cells - '''key feature'''.
**May be sterile, i.e. no organisms.
**May be sterile, i.e. no organisms.
*+/-Fibrosis.
*+/-Necrotic bone - bone with empty lacunae.
*+/-[[Amyloid]].
**~10% of patients in one series.<ref name=pmid2071276>{{Cite journal  | last1 = Alabi | first1 = ZO. | last2 = Ojo | first2 = OS. | last3 = Odesanmi | first3 = WO. | title = Secondary amyloidosis in chronic osteomyelitis. | journal = Int Orthop | volume = 15 | issue = 1 | pages = 21-2 | month =  | year = 1991 | doi =  | PMID = 2071276 }}</ref>
Image:
*[http://osteomyelitis.stanford.edu/pages/histo_001.html Chronic osteomyelitis (stanford.edu)].
===Sign out===
<pre>
LOWER LEG, RIGHT, BELOW KNEE AMPUTATION:
- ACUTE AND CHRONIC OSTEOMYELITIS.
- MODERATE ATHEROSCLEROSIS.
- SKIN ULCERATION.
- SOFT TISSUE SURGICAL MARGIN WITH FAT NECROSIS AND MUSCULAR ATROPHY.
- NEGATIVE FOR MALIGNANCY.
</pre>
<pre>
TRAPEZIUM, RIGHT HAND, REMOVAL:
- DEGENERATIVE JOINT DISEASE.
- MARROW SPACE WITH FIBROSIS AND SCATTERED LYMPHOCYTES AND PLASMA CELLS,
  COMPATIBLE WITH MILD CHRONIC OSTEOMYELITIS.
- NO MICROORGANISMS APPARENT WITH ROUTINE STAINS.
- NEGATIVE FOR MALIGNANCY.
</pre>


=Bone tumours=
=Bone tumours=
Line 86: Line 175:


=Fractures=
=Fractures=
{{Main|Forensic pathology}}
{{Main|Bone fractures}}
This is dealt with in the ''forensic pathology'' article.
This is dealt with in the ''forensic pathology'' article.


Line 94: Line 183:
A general DDx for cystic bone lesions is found on ''radipedia.com''.<ref>URL: [http://radipedia.com/WikiMedia/index.php?title=Benign_cystic_bone_lesions http://radipedia.com/WikiMedia/index.php?title=Benign_cystic_bone_lesions]. Accessed on: 15 March 2011.</ref>
A general DDx for cystic bone lesions is found on ''radipedia.com''.<ref>URL: [http://radipedia.com/WikiMedia/index.php?title=Benign_cystic_bone_lesions http://radipedia.com/WikiMedia/index.php?title=Benign_cystic_bone_lesions]. Accessed on: 15 March 2011.</ref>


==Aneurysmal bone cyst==
==Sequestrum==
*Abbreviated ''ABC''.
{{Main|Sequestrum}}


===General===
==Osteoarthritis==
Features:<ref name=emed_abc>URL: [http://emedicine.medscape.com/article/1254784-overview http://emedicine.medscape.com/article/1254784-overview]. Accessed on: 4 February 2011.</ref>
{{Main|Osteoarthritis}}
*Benign.
This keeps orthopaedic surgeons busy.
**May grow rapidly.
*Osteolysis -> cystic space -> filled with blood.
*Relatively common; in children second only to osteosarcoma.<ref>{{cite journal |author=van den Berg H, Kroon HM, Slaar A, Hogendoorn P |title=Incidence of biopsy-proven bone tumors in children: a report based on the Dutch pathology registration "PALGA" |journal=J Pediatr Orthop |volume=28 |issue=1 |pages=29–35 |year=2008 |pmid=18157043 |doi=10.1097/BPO.0b013e3181558cb5 |url=}}</ref>


===Microscopic===
==Aneurysmal bone cyst==
Features:<ref name=emed_abc/>
{{Main|Aneurysmal bone cyst}}
*Bony trabeculae or osteoid tissue.
*Osteoclast giant cells.
**Multi-nucleated giant-cells with round randomly arranged nuclei.
*Fibroblasts - surround bone.
 
DDx:
*[[Giant cell tumour of bone]].
*Telangiectatic [[osteosarcoma]].


==Myositis ossificans==
==Myositis ossificans==
Line 138: Line 216:
**Lesion is well-circumscribed.
**Lesion is well-circumscribed.
**Normal muscle is adjacent to the lesion - '''key feature'''.
**Normal muscle is adjacent to the lesion - '''key feature'''.
DDx:
*[[Heterotopic ossification]] - bone, not cellular, no inflammation.
Images:
*[http://path.upmc.edu/cases/case72/micro.html Myositis ossificans (upmc.edu)].


==Paget disease of the bone==
==Paget disease of the bone==
{{Main|Paget's disease of the bone}}
==Fibrous dysplasia==
*[[AKA]] ''osteitis fibrosa''.
{{Main|Fibrous dysplasia}}
==Desmoplastic fibroma==
*'''Not''' to be confused with ''[[desmoplastic fibroblastoma]]''.
===General===
===General===
*Benign - unlike [[Paget disease of the breast]].
*Rare.


Classically divided into three phases:<ref name=emed_paget>URL: [http://emedicine.medscape.com/article/311688-overview http://emedicine.medscape.com/article/311688-overview]. Accessed on: 25 December 2010.</ref><ref>URL: [http://radiopaedia.org/articles/paget-disease-of-bone-1 http://radiopaedia.org/articles/paget-disease-of-bone-1]. Accessed on: 25 December 2010.</ref>
===Microscopic===
#Lytic (predominantly osteoclasts).  
Features:<ref>URL: [http://www.bonetumor.org/tumors-fibrous-tissue/desmoplastic-fibroma http://www.bonetumor.org/tumors-fibrous-tissue/desmoplastic-fibroma]. Accessed on: 14 April 2011.</ref>
#Mixed lytic (osteoclastic) and blastic (osteoblastic).
*Lamellar bone.
#Sclerotic (burned-out).
*Fibrotic marrow space with:
**Collagen.
**Low cellularity.
**Spindle cells without significant atypia.


Clinical:
DDx:
*Elevated ALP.
*[[Fibrous dysplasia]] - has woven bone.
*Low grade [[fibrosarcoma]].


==Gaucher disease==
{{Main|Gaucher disease}}
===General===
*May present as a fracture.
===Microscopic===
===Microscopic===
Features:<ref name=emed_paget>URL: [http://emedicine.medscape.com/article/311688-overview http://emedicine.medscape.com/article/311688-overview]. Accessed on: 25 December 2010.</ref>
*Macrophages in the marrow space with a "crumpled tissue paper" appearance.
*Bone matrix has jigsaw-puzzle like pattern.
**Jigsaw-puzzle pieces each ~ 100-500 micrometres in size (largest dimension).
*Increased osteoclast activity.
**Osteoclast = macrophage that resorbs bone matrix.


Images:
==Langerhans cell histiocytosis of bone==
*[http://commons.wikimedia.org/wiki/File:Paget_disease_of_bone_-_high_mag.jpg Paget disease - bone - high mag. (WC)].
*[[AKA]] ''eosinophilic granuloma of bone''.
*[http://commons.wikimedia.org/wiki/File:Paget_disease_of_bone_-_intermed_mag.jpg Paget disease - bone - intermed. mag. (WC)].
{{Main|Langerhans cell histiocytosis}}
===General===
*Rare.
*Children.
===Microscopic===
Features:
*Eosinophils.
*Cerebriform and/or reniform macrophages.


==Fibrous dysplasia==
==Giant cell reparative granuloma==
*[[AKA]] ''osteitis fibrosa''.
*[[AKA]] ''central [[giant cell]] granuloma'',<ref name=ijri>Shah UA, Shah AK, Kumar S. Giant cell reparative granuloma of the jaw: A case report. Indian J Radiol Imaging [serial online] 2006 [cited 2012 Feb 28];16:677-8. Available from: [http://www.ijri.org/text.asp?2006/16/4/677/32297 http://www.ijri.org/text.asp?2006/16/4/677/32297].</ref> abbreviated ''CGCG''.
*[[AKA]] ''solid [[aneurysmal bone cyst]]''.<ref name=pmid22285042>{{Cite journal  | last1 = Pan | first1 = Z. | last2 = Sanger | first2 = WG. | last3 = Bridge | first3 = JA. | last4 = Hunter | first4 = WJ. | last5 = Siegal | first5 = GP. | last6 = Wei | first6 = S. | title = A novel t(6;13)(q15;q34) translocation in a giant cell reparative granuloma (solid aneurysmal bone cyst). | journal = Hum Pathol | volume =  | issue =  | pages =  | month = Jan | year = 2012 | doi = 10.1016/j.humpath.2011.10.003 | PMID = 22285042 }}</ref>
===General===
===General===
Classification:
*Lesion of the mandible and maxilla.<ref name=ijri/>
*''Monostotic'' - one bone involved, ~80% of cases.
 
*''Polyostotic'' - several bones involved, ~20% of cases.
===Radiology===
**May be associated with [[McCune-Albright syndrome]].
*Lytic lesion.


===Microscopic===
===Microscopic===
Features:<ref>URL: [[http://www.pathologypics.com/pictview.aspx?id=104 http://www.pathologypics.com/pictview.aspx?id=104]. Accessed on: 14 April 2011.</ref>
Features:<ref name=ijri/>
*Woven bone with odd irregular shapes.
*Giant cells.
**Described as "chinese characters".  
*Fibroblasts.
*Fibrous tissue around bone.
*Osteoid.
*Hemosiderin-laden macrophages.


Notes:
DDx:
*No osteoblastic rimming.
*Peripheral giant cell granuloma - soft tissue counterpart of CGCG.
*[[Giant cell tumour of bone]].
 
====Images====
<gallery>
Image:Central_giant_cell_granuloma_-_intermed_mag.jpg | CGCG - intermed. mag. (WC/Nephron)
Image:Central_giant_cell_granuloma_-_high_mag.jpg | CGCG - high mag. (WC/Nephron)
</gallery>
===Molecular===
Recurrent [[chromosomal translocation]]:<ref name=pmid22285042/>
*t(16;17)(q22;p13).
 
==Osteopetrosis==
*[[AKA]] ''marble bone disease''.<ref name=pmid19232111>{{Cite journal  | last1 = Stark | first1 = Z. | last2 = Savarirayan | first2 = R. | title = Osteopetrosis. | journal = Orphanet J Rare Dis | volume = 4 | issue =  | pages = 5 | month =  | year = 2009 | doi = 10.1186/1750-1172-4-5 | PMID = 19232111 | PMC = 2654865 }}</ref>
===General===
*Rare.
*Genetic - may be autosomal dominant, autosomal recessive or X-linked.<ref name=pmid19232111/>
*Pancytopenias - due to oblieration of the marrow space.
*[[bone fractures|Fractures]].
*Radiologic diagnosis.
 
===Gross===
*Marbled appearance.
*Thickening with obliteration of the marrow space.
*Patchy (brown) surface due to vascularization/[[extramedullary hematopoiesis]].<ref name=Ref_AoGP469>{{Ref AoGP|469}}</ref>
 
Image:
*[http://www.uaz.edu.mx/histo/pathology/ed/ch_26/c26_s25.htm Osteopetrosis (uaz.edu.mx)].
 
===Microscopic===
Features:<ref name=pmid11264157/>
*Abundant irregular (pink) bony trabeculae with layers of (blue-gray) [[cartilage]].


Image:
Image:
*[http://www.pathologypics.com/pictview.aspx?id=104 Fibrous dysplasia of bone - high mag. (pathologypics.com)].
*[http://bloodjournal.hematologylibrary.org/content/97/7/1947/F2.expansion.html Osteopetrosis (bloodjournal.hematologylibrary.org)].<ref name=pmid11264157>{{Cite journal  | last1 = McMahon | first1 = C. | last2 = Will | first2 = A. | last3 = Hu | first3 = P. | last4 = Shah | first4 = GN. | last5 = Sly | first5 = WS. | last6 = Smith | first6 = OP. | title = Bone marrow transplantation corrects osteopetrosis in the carbonic anhydrase II deficiency syndrome. | journal = Blood | volume = 97 | issue = 7 | pages = 1947-50 | month = Apr | year = 2001 | doi =  | PMID = 11264157 |URL = http://bloodjournal.hematologylibrary.org/content/97/7/1947.full }}</ref>
*[http://www.pathologypics.com/BigPicViewer.aspx?PictureID=103 Fibrous dysplasia of bone - low mag. (pathologypics.com)].
 
==Osteoporosis==
===General===
*Very common.
*Associated with [[bone fractures|fractures]], esp. wrist, [[traumatic hip fracture|hip]] & vertebra.<ref name=pmid22537071>{{Cite journal  | last1 = Lix | first1 = LM. | last2 = Azimaee | first2 = M. | last3 = Acan Osman | first3 = B. | last4 = Caetano | first4 = P. | last5 = Morin | first5 = S. | last6 = Metge | first6 = C. | last7 = Goltzman | first7 = D. | last8 = Kreiger | first8 = N. | last9 = Prior | first9 = J. | title = Osteoporosis-related fracture case definitions for population-based administrative data. | journal = BMC Public Health | volume = 12 | issue = 1 | pages = 301 | month = Apr | year = 2012 | doi = 10.1186/1471-2458-12-301 | PMID = 22537071 }}</ref>
*Radiologic diagnosis - bone mineral density.
 
Etiology:<ref name=Ref_PCPBoD8_617>{{Ref PCPBoD8|617}}</ref>
*Primary (senile).
*Secondary:
**Endocrine:
***Hyperparathyroidism.
**Gastrointestinal:
***Malabsorption, e.g. [[celiac disease]], [[cystic fibrosis]], [[Crohn's disease]].
***Starvation.
**Psychiatric:
***Anorexia nervosa.
***Bulimia nervosa.
**Neoplasia:
***[[Multiple myeloma]].
**Drugs:
***Systemic steroids.<ref name=pmid16243531>{{Cite journal  | last1 = Dalle Carbonare | first1 = L. | last2 = Bertoldo | first2 = F. | last3 = Valenti | first3 = MT. | last4 = Zenari | first4 = S. | last5 = Zanatta | first5 = M. | last6 = Sella | first6 = S. | last7 = Giannini | first7 = S. | last8 = Cascio | first8 = VL. | title = Histomorphometric analysis of glucocorticoid-induced osteoporosis. | journal = Micron | volume = 36 | issue = 7-8 | pages = 645-52 | month =  | year = 2005 | doi = 10.1016/j.micron.2005.07.009 | PMID = 16243531 }}</ref>
 
===Gross===
*Decreased numbers of, abnormally thin, bony spicules.
 
Images:
*[http://www.sciencephoto.com/media/260363/enlarge Osteoporosis (sciencephoto.com)].
*[http://library.med.utah.edu/WebPath/TUTORIAL/OSTEO/OST005.html Osteoporosis (utah.edu)].
 
===Microscopic===
Features (femoral neck):<ref name=pmid18708176>{{Cite journal  | last1 = Blain | first1 = H. | last2 = Chavassieux | first2 = P. | last3 = Portero-Muzy | first3 = N. | last4 = Bonnel | first4 = F. | last5 = Canovas | first5 = F. | last6 = Chammas | first6 = M. | last7 = Maury | first7 = P. | last8 = Delmas | first8 = PD. | title = Cortical and trabecular bone distribution in the femoral neck in osteoporosis and osteoarthritis. | journal = Bone | volume = 43 | issue = 5 | pages = 862-8 | month = Nov | year = 2008 | doi = 10.1016/j.bone.2008.07.236 | PMID = 18708176 }}</ref>
*Thinner cortex.
*Decreased trabecular thickness
*Decreased number of trabeculae.
 
Note:
*One study looking at iliac bone biopsies suggests trabeculae are no different in osteoporosis.<ref name=pmid3414359>{{Cite journal  | last1 = Chappard | first1 = D. | last2 = Alexandre | first2 = C. | last3 = Riffat | first3 = G. | title = Spatial distribution of trabeculae in iliac bone from 145 osteoporotic females. | journal = Acta Anat (Basel) | volume = 132 | issue = 2 | pages = 137-42 | month =  | year = 1988 | doi =  | PMID = 3414359 }}</ref>


=See also=
=See also=
*[[Cartilage]].
*[[Chondro-osseous tumours]].
*[[Femoral head]].
*[[Spine]].
*[[Hematopathology]].
*[[Hematopathology]].
*[[Soft tissue lesions]].
*[[Soft tissue lesions]].
*[[Small round cell tumours]].
*[[Small round cell tumours]].
*[[Chondro-osseous tumours]].
*[[Cartilage]].


=References=
=References=
{{reflist|2}}
{{reflist|2}}


[[Category:Weird stuff]]
=External links=
*[http://www.medicalhistology.us/twiki/bin/view/Main/CartilageAndBoneAtlas08 Bone and cartilage histology (medicalhistology.us)].
 
[[Category:Bone]]

Latest revision as of 21:39, 23 June 2018

Bone is a scaffold it bears weight and occasionally gets infected.

Tumours often spread to bone and occasionally arise in bone. Bone tumours are dealt with in the bone tumours article.

Normal bone

Bone anatomy

  • Epiphysis = rounded end of the bone.
  • Metaphysis = between epiphysis and diaphysis; contains epiphyseal plate - site of growth in childhood.
  • Diaphysis = shaft, mid-portion, contains bone marrow.

Image

  • Bone. (WC)

Bone histology

Two types (based on arrangement of collagen):

  1. Woven bone.
  2. Lamellar bone.

Woven bone

  • Always abnormal in adults.
  • Collagen arranged haphazardly - mechanically weak.

Images:

Lamellar bone

Animated GIF showing a photomicrograph of lamellar bone. The layers are accentuated under polarised light.
  • Collagen organized in layers (lamellae).

Woven versus lamellar

  • Easiest way to differentiate: polarize; lamellar bone has well-defined layers.

Bone cells

  • Osteocytes.
    • Sit in lacunae.
      • Empty lacunae = necrotic bone.
  • Osteoblasts.
    • Make bone.
  • Osteoclasts.
    • Destroy bone.
    • Multinucleated.

Memory device: 'b' before 'c'.

Bone marrow

Main article: Haematopoiesis

General

Bone marrow biopsy adequacy:[3]

  • 10 intertrabecular spaces or 10 mm.

Microscopic

One should see three cell lines:[4]

  1. Erythroid (red cells).
  2. Myeloid (white blood cells).
  3. Megakaryocytic (platelets).

Adipose tissue:

  • Fat content (%) ~= age in years.[5]
    • e.g. 60 year old will have 60% fatty replacement.

Identifying the cell lines:[6]

  1. Megakaryocytes:
    • Big cells ~ 3x the size of a RBC.
  2. Normoblasts (RBC precursors):
    • Hyperchromatic, i.e. blue, nucleus.
  3. Myeloid line:
    • Granules.
    • Reniform nucleus, i.e. kidney bean shaped nucleus.

Note:

  • Lymphocytes are considered separately and typically spared in bone marrow failure.[7]

Organization

  • Mature hematopoeitic cells at the centre (distant from bone).
  • Immature hematopoeitic cells adjacent to the bone.

Note:

Images

  • Myeloid line. (WC)

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Essentially normal

Bone Marrow, Posterior Superior Iliac Spine, Biopsy:
- Bone marrow with trilineage hematopoiesis.
- Cellularity within normal limits for age.
- Case will be sent to hematology for consultation and correlation 
  with other testing.

Inadequate

Right Posterior Superior Iliac Spine, Bone Marrow Biopsy:
- Predominantly benign dense fibrous tissue with a small 
  amount of laminar bone, and minute fragment of marrow (<2 mm).
- Case will be sent to hematopathology for further assessment.

Benign variants

Hyperostosis frontalis interna

  • Extra-thick frontal bone.[9]
  • No clinical significance -- just has to be recognized as a "nothing".

Infections

Acute osteomyelitis

General

Pathophysiology/entry:

  • Hematogenous - often in children.
  • Direct entry (skin defect) - adults with diabetes.

Microscopic

Features:

  • PMNs.
  • Micro-organisms - esp. cocci.

Image:

Stains

Chronic osteomyelitis

General

Most common organism:

  • Staphylococcus aureus.[10]

Risk factors:[10]

Microscopic

Features:

  • Plasma cells - key feature.
    • May be sterile, i.e. no organisms.
  • +/-Fibrosis.
  • +/-Necrotic bone - bone with empty lacunae.
  • +/-Amyloid.
    • ~10% of patients in one series.[11]

Image:

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LOWER LEG, RIGHT, BELOW KNEE AMPUTATION:
- ACUTE AND CHRONIC OSTEOMYELITIS.
- MODERATE ATHEROSCLEROSIS.
- SKIN ULCERATION.
- SOFT TISSUE SURGICAL MARGIN WITH FAT NECROSIS AND MUSCULAR ATROPHY.
- NEGATIVE FOR MALIGNANCY.

TRAPEZIUM, RIGHT HAND, REMOVAL:
- DEGENERATIVE JOINT DISEASE.
- MARROW SPACE WITH FIBROSIS AND SCATTERED LYMPHOCYTES AND PLASMA CELLS,
  COMPATIBLE WITH MILD CHRONIC OSTEOMYELITIS.
- NO MICROORGANISMS APPARENT WITH ROUTINE STAINS.
- NEGATIVE FOR MALIGNANCY.

Bone tumours

Main article: Bone tumours

This is a big topic. It is dealt with in a separate article.

The bone tumour article covers tumour mimics, e.g. brown cell tumour.

Fractures

Main article: Bone fractures

This is dealt with in the forensic pathology article.

Others

The following is a collection of stuff that doesn't really fit in another category or is just weird.

A general DDx for cystic bone lesions is found on radipedia.com.[12]

Sequestrum

Main article: Sequestrum

Osteoarthritis

Main article: Osteoarthritis

This keeps orthopaedic surgeons busy.

Aneurysmal bone cyst

Main article: Aneurysmal bone cyst

Myositis ossificans

  • AKA fibro-osseous pseudotumour of digits.[13]

General

Epidemiology:

  • Young people.
  • History of trauma - typically.
  • Extremities - digits (fingers, toes).

Notes:

  • Histologically "worrisome" (for malignancy) - due to high cellularity.[13]

Microscopic

Features:[13]

  • High cellularity.
  • Low mitotic activity.
  • No atypical mitoses.
  • No hyperchromasia.

Other features:[14]

  • Low power diagnosis:
    • Lesion is well-circumscribed.
    • Normal muscle is adjacent to the lesion - key feature.

DDx:

Images:

Paget disease of the bone

Main article: Paget's disease of the bone

Fibrous dysplasia

  • AKA osteitis fibrosa.
Main article: Fibrous dysplasia

Desmoplastic fibroma

General

  • Rare.

Microscopic

Features:[15]

  • Lamellar bone.
  • Fibrotic marrow space with:
    • Collagen.
    • Low cellularity.
    • Spindle cells without significant atypia.

DDx:

Gaucher disease

Main article: Gaucher disease

General

  • May present as a fracture.

Microscopic

  • Macrophages in the marrow space with a "crumpled tissue paper" appearance.

Langerhans cell histiocytosis of bone

  • AKA eosinophilic granuloma of bone.
Main article: Langerhans cell histiocytosis

General

  • Rare.
  • Children.

Microscopic

Features:

  • Eosinophils.
  • Cerebriform and/or reniform macrophages.

Giant cell reparative granuloma

General

  • Lesion of the mandible and maxilla.[16]

Radiology

  • Lytic lesion.

Microscopic

Features:[16]

  • Giant cells.
  • Fibroblasts.
  • Osteoid.
  • Hemosiderin-laden macrophages.

DDx:

Images

  • CGCG - intermed. mag. (WC/Nephron)

  • CGCG - high mag. (WC/Nephron)

Molecular

Recurrent chromosomal translocation:[17]

  • t(16;17)(q22;p13).

Osteopetrosis

General

  • Rare.
  • Genetic - may be autosomal dominant, autosomal recessive or X-linked.[18]
  • Pancytopenias - due to oblieration of the marrow space.
  • Fractures.
  • Radiologic diagnosis.

Gross

Image:

Microscopic

Features:[20]

  • Abundant irregular (pink) bony trabeculae with layers of (blue-gray) cartilage.

Image:

Osteoporosis

General

  • Very common.
  • Associated with fractures, esp. wrist, hip & vertebra.[21]
  • Radiologic diagnosis - bone mineral density.

Etiology:[22]

Gross

  • Decreased numbers of, abnormally thin, bony spicules.

Images:

Microscopic

Features (femoral neck):[24]

  • Thinner cortex.
  • Decreased trabecular thickness
  • Decreased number of trabeculae.

Note:

  • One study looking at iliac bone biopsies suggests trabeculae are no different in osteoporosis.[25]

See also

References

  1. ↑ Lin DD, Gailloud P, McCarthy EF, Comi AM (February 2006). "Oromaxillofacial osseous abnormality in Sturge-Weber syndrome: case report and review of the literature". AJNR Am J Neuroradiol 27 (2): 274–7. PMID 16484391.
  2. ↑ URL: http://www.life.umd.edu/classroom/bsci423/song/Lab1.html. Accessed on: 28 March 2012.
  3. ↑ Rudzki, Z.; Partyła, T.; Okoń, K.; Stachura, J. (2005). "Adequacy of trephine bone marrow biopsies: the doctor and the patient make a difference.". Pol J Pathol 56 (4): 187-95. PMID 16477878.
  4. ↑ URL: http://emedicine.medscape.com/article/199003-overview. Accessed on: 28 March 2012.
  5. ↑ IAV. 26 Feb 2009.
  6. ↑ http://upload.wikimedia.org/wikipedia/commons/6/69/Hematopoiesis_%28human%29_diagram.png
  7. ↑ http://emedicine.medscape.com/article/199003-overview
  8. ↑ Iancu, D.; Hao, S.; Lin, P.; Anderson, SK.; Jorgensen, JL.; McLaughlin, P.; Medeiros, LJ. (Feb 2007). "Follicular lymphoma in staging bone marrow specimens: correlation of histologic findings with the results of flow cytometry immunophenotypic analysis.". Arch Pathol Lab Med 131 (2): 282-7. doi:10.1043/1543-2165(2007)131[282:FLISBM]2.0.CO;2. PMID 17284114.
  9. ↑ URL: http://radiopaedia.org/articles/hyperostosis_frontalis_interna. Accessed on: 29 September 2010.
  10. ↑ 10.0 10.1 Hatzenbuehler, J.; Pulling, TJ. (Nov 2011). "Diagnosis and management of osteomyelitis.". Am Fam Physician 84 (9): 1027-33. PMID 22046943.
  11. ↑ Alabi, ZO.; Ojo, OS.; Odesanmi, WO. (1991). "Secondary amyloidosis in chronic osteomyelitis.". Int Orthop 15 (1): 21-2. PMID 2071276.
  12. ↑ URL: http://radipedia.com/WikiMedia/index.php?title=Benign_cystic_bone_lesions. Accessed on: 15 March 2011.
  13. ↑ 13.0 13.1 13.2 Humphrey, Peter A; Dehner, Louis P; Pfeifer, John D (2008). The Washington Manual of Surgical Pathology (1st ed.). Lippincott Williams & Wilkins. pp. 607. ISBN 978-0781765275.
  14. ↑ IAV. 9 December 2010.
  15. ↑ URL: http://www.bonetumor.org/tumors-fibrous-tissue/desmoplastic-fibroma. Accessed on: 14 April 2011.
  16. ↑ 16.0 16.1 16.2 Shah UA, Shah AK, Kumar S. Giant cell reparative granuloma of the jaw: A case report. Indian J Radiol Imaging [serial online] 2006 [cited 2012 Feb 28];16:677-8. Available from: http://www.ijri.org/text.asp?2006/16/4/677/32297.
  17. ↑ 17.0 17.1 Pan, Z.; Sanger, WG.; Bridge, JA.; Hunter, WJ.; Siegal, GP.; Wei, S. (Jan 2012). "A novel t(6;13)(q15;q34) translocation in a giant cell reparative granuloma (solid aneurysmal bone cyst).". Hum Pathol. doi:10.1016/j.humpath.2011.10.003. PMID 22285042.
  18. ↑ 18.0 18.1 Stark, Z.; Savarirayan, R. (2009). "Osteopetrosis.". Orphanet J Rare Dis 4: 5. doi:10.1186/1750-1172-4-5. PMC 2654865. PMID 19232111. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2654865/.
  19. ↑ Rose, Alan G. (2008). Atlas of Gross Pathology with Histologic Correlation (1st ed.). Cambridge University Press. pp. 469. ISBN 978-0521868792.
  20. ↑ 20.0 20.1 McMahon, C.; Will, A.; Hu, P.; Shah, GN.; Sly, WS.; Smith, OP. (Apr 2001). "Bone marrow transplantation corrects osteopetrosis in the carbonic anhydrase II deficiency syndrome.". Blood 97 (7): 1947-50. PMID 11264157.
  21. ↑ Lix, LM.; Azimaee, M.; Acan Osman, B.; Caetano, P.; Morin, S.; Metge, C.; Goltzman, D.; Kreiger, N. et al. (Apr 2012). "Osteoporosis-related fracture case definitions for population-based administrative data.". BMC Public Health 12 (1): 301. doi:10.1186/1471-2458-12-301. PMID 22537071.
  22. ↑ Mitchell, Richard; Kumar, Vinay; Fausto, Nelson; Abbas, Abul K.; Aster, Jon (2011). Pocket Companion to Robbins & Cotran Pathologic Basis of Disease (8th ed.). Elsevier Saunders. pp. 617. ISBN 978-1416054542.
  23. ↑ Dalle Carbonare, L.; Bertoldo, F.; Valenti, MT.; Zenari, S.; Zanatta, M.; Sella, S.; Giannini, S.; Cascio, VL. (2005). "Histomorphometric analysis of glucocorticoid-induced osteoporosis.". Micron 36 (7-8): 645-52. doi:10.1016/j.micron.2005.07.009. PMID 16243531.
  24. ↑ Blain, H.; Chavassieux, P.; Portero-Muzy, N.; Bonnel, F.; Canovas, F.; Chammas, M.; Maury, P.; Delmas, PD. (Nov 2008). "Cortical and trabecular bone distribution in the femoral neck in osteoporosis and osteoarthritis.". Bone 43 (5): 862-8. doi:10.1016/j.bone.2008.07.236. PMID 18708176.
  25. ↑ Chappard, D.; Alexandre, C.; Riffat, G. (1988). "Spatial distribution of trabeculae in iliac bone from 145 osteoporotic females.". Acta Anat (Basel) 132 (2): 137-42. PMID 3414359.

External links

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