Flow cytometry - Revision history

Michael: more

2012-12-27T11:10:20Z

← Older revision		Revision as of 11:10, 27 December 2012
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	# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref>) and interrogated with a laser light.		# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref>) and interrogated with a laser light.

	==Scatter of light==		===Scatter of light===
	Types:		Types:
	#Forward scatter (FS) -- indicative of cell size.		#Forward scatter (FS) -- indicative of cell size.
	#Side scatter (SS) -- indicative of cell complexity (granularity).		#Side scatter (SS) -- indicative of cell complexity (granularity).

	===Scatter in a table===		====Scatter in a table====
	Selected white blood cells & scatter:<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif]. Accessed on: 5 August 2010.</ref>		Selected white blood cells & scatter:<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif]. Accessed on: 5 August 2010.</ref>
	{\| class="wikitable"		{\| class="wikitable"
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	\| low		\| low
	\|}		\|}

			==Practical considerations==
			*Tissue must be fresh, i.e. cannot be [[formalin]] fixed.
			**Viable material may be salvaged from the centre of a large specimen placed in formalin for a short time.
			*2 mm x 2 mm x 2 mm piece of tissue is sufficient.
			**1 mm<sup>3</sup> is considered the minimum by Mayo Medical Laboratories.<ref>URL: [http://www.mayomedicallaboratories.com/test-catalog/Specimen/19499 http://www.mayomedicallaboratories.com/test-catalog/Specimen/19499]. Accessed on: 27 December 2012.</ref>

	==Interpretation==		==Interpretation==

Michael: +interpretation

2011-05-27T14:46:15Z

+interpretation

← Older revision		Revision as of 14:46, 27 May 2011
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			==Interpretation==
			:See ''[[Cytometry]]''.

	==See also==		==See also==

Michael: table

2010-08-05T20:49:57Z

table

← Older revision		Revision as of 20:49, 5 August 2010
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	==Principles==		==Principles==
	# Label cells with fluorescent antibodies.		# Label cells with fluorescent antibodies.
	# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref> -- interrogated with a laser light.		# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref>) and interrogated with a laser light.

			==Scatter of light==
			Types:
			#Forward scatter (FS) -- indicative of cell size.
			#Side scatter (SS) -- indicative of cell complexity (granularity).

			===Scatter in a table===
			Selected white blood cells & scatter:<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/images/blood.gif]. Accessed on: 5 August 2010.</ref>
			{\| class="wikitable"
			!
			! Forward scatter
			! Side scatter
			\|-
			\| PMNs
			\| high
			\| very high
			\|-
			\| Monocytes
			\| high
			\| moderate/low
			\|-
			\| Lymphocytes
			\| low
			\| low
			\|}

	==See also==		==See also==

Michael: create

2010-08-05T20:40:28Z

create

New page

'''Flow cytometry''' is an automated technique used to examine cells that is widely used for the diagnosis and characterization of [[lymphoma]].

==Principles==
# Label cells with fluorescent antibodies.
# Passed through a channel one-at-a-time (using ''hydrodynamic focusing''<ref>URL: [http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm]. Accessed on: 5 August 2010.</ref> -- interrogated with a laser light.

==See also==
*[[Haematopathology]].
*[[Cytopathology]].

==References==
{{reflist|2}}

==External links==
*[http://facs.stanford.edu/sff/about-facs.html Flow cytometry (standford.edu)].
*[http://www.med.umich.edu/flowcytometry/InitialTraining/lessons/lesson1/index.htm Flow cytometry (med.umich.edu)].

[[Category:Haematopathology]]