Anatomical pathology laboratory processes - Revision history

Michael at 21:08, 27 December 2023

2023-12-27T21:08:19Z

← Older revision		Revision as of 21:08, 27 December 2023
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	**Routine sections are cut at 3-4 micrometres.		**Routine sections are cut at 3-4 micrometres.
	***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>		***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>
	**Sections for [[Congo red\|Congo red]] are usually ~10 micrometres.		***4 micrometres is a recommendation for immunostains.<ref>{{cite journal \|authors=Magaki S, Hojat SA, Wei B, So A, Yong WH \|title=An Introduction to the Performance of Immunohistochemistry \|journal=Methods Mol Biol \|volume=1897 \|issue= \|pages=289–298 \|date=2019 \|pmid=30539453 \|pmc=6749998 \|doi=10.1007/978-1-4939-8935-5_25 \|url=}}</ref>
	**4 micrometres is a recommendation for immunostains.<ref>{{cite journal \|authors=Magaki S, Hojat SA, Wei B, So A, Yong WH \|title=An Introduction to the Performance of Immunohistochemistry \|journal=Methods Mol Biol \|volume=1897 \|issue= \|pages=289–298 \|date=2019 \|pmid=30539453 \|pmc=6749998 \|doi=10.1007/978-1-4939-8935-5_25 \|url=}}</ref>		***Sections for [[Congo red\|Congo red]] are usually ~10 micrometres.


	===Staining===		===Staining===

Michael at 21:07, 27 December 2023

2023-12-27T21:07:34Z

← Older revision		Revision as of 21:07, 27 December 2023
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	***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>		***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>
	**Sections for [[Congo red\|Congo red]] are usually ~10 micrometres.		**Sections for [[Congo red\|Congo red]] are usually ~10 micrometres.
			**4 micrometres is a recommendation for immunostains.<ref>{{cite journal \|authors=Magaki S, Hojat SA, Wei B, So A, Yong WH \|title=An Introduction to the Performance of Immunohistochemistry \|journal=Methods Mol Biol \|volume=1897 \|issue= \|pages=289–298 \|date=2019 \|pmid=30539453 \|pmc=6749998 \|doi=10.1007/978-1-4939-8935-5_25 \|url=}}</ref>

	===Staining===		===Staining===

Michael: /* Processes */

2015-12-23T13:44:13Z

Processes

← Older revision		Revision as of 13:44, 23 December 2015
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	#***Immunostains.		#***Immunostains.
	#***[[Sign out\|Write report]].		#***[[Sign out\|Write report]].
	#****Consult other pathologists - in house of outside.		#****Consult other pathologists - in house (internal consult) or outside (external consult).
	#****Discuss with clinician.		#****Discuss with clinician.

Michael: /* Processes */

2015-06-08T23:05:04Z

Processes

← Older revision		Revision as of 23:05, 8 June 2015
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	#Staining.		#Staining.
	#*Routinue stains.		#*Routinue stains.
	#*Special stains.		#*[[Special stains]].
	#*[[Immunostains]].		#*[[Immunostains]].
	#Case assembly and distribution.		#Case assembly and distribution.
	#Diagnosis.		#Diagnosis.
	#*Pathologist.		#*Pathologist +/- pathology [[resident]].
	#**Tasks:		#**Tasks:
	#***More tissue.		#***More tissue.
	#***Levels/deepers.		#***[[Levels]]/deepers.
	#***Special [[stains]].		#***Special [[stains]].
	#***Immunostains.		#***Immunostains.
	#***Write report.		#***[[Sign out\|Write report]].
	#****Consult other pathologists - in house of outside.		#****Consult other pathologists - in house of outside.
	#****Discuss with clinician.		#****Discuss with clinician.

Michael: /* External links */

2015-01-20T17:54:02Z

External links

← Older revision		Revision as of 17:54, 20 January 2015
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	*[[Fixation]].		*[[Fixation]].
	*[[Laboratory information system]].		*[[Laboratory information system]].

			==References==
			{{Reflist\|1}}

	==External links==		==External links==

Michael: /* Cutting */

2015-01-20T17:53:38Z

Cutting

← Older revision		Revision as of 17:53, 20 January 2015
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	**Who cut the tissue is tracked.		**Who cut the tissue is tracked.

	~~Note~~:		Notes:
	*The thickness of the sections can make a difference in the interpretation.<ref name=pmid1915756>{{Cite journal \| last1 = Otto \| first1 = MJ. \| last2 = Löw \| first2 = O. \| last3 = Schneider \| first3 = A. \| title = The nominal section thickness--importance of their correction for morphometry. \| journal = Exp Pathol \| volume = 42 \| issue = 3 \| pages = 129-36 \| month = \| year = 1991 \| doi = \| PMID = 1915756 }}</ref>		*The thickness of the sections can make a difference in the interpretation.<ref name=pmid1915756>{{Cite journal \| last1 = Otto \| first1 = MJ. \| last2 = Löw \| first2 = O. \| last3 = Schneider \| first3 = A. \| title = The nominal section thickness--importance of their correction for morphometry. \| journal = Exp Pathol \| volume = 42 \| issue = 3 \| pages = 129-36 \| month = \| year = 1991 \| doi = \| PMID = 1915756 }}</ref>
	**Routine sections are cut at 3-4 micrometres.		**Routine sections are cut at 3-4 micrometres.
	***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>		***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>
			**Sections for [[Congo red\|Congo red]] are usually ~10 micrometres.

	===Staining===		===Staining===

Michael: /* Cutting */

2015-01-20T17:51:55Z

Cutting

← Older revision		Revision as of 17:51, 20 January 2015
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	*The thickness of the sections can make a difference in the interpretation.<ref name=pmid1915756>{{Cite journal \| last1 = Otto \| first1 = MJ. \| last2 = Löw \| first2 = O. \| last3 = Schneider \| first3 = A. \| title = The nominal section thickness--importance of their correction for morphometry. \| journal = Exp Pathol \| volume = 42 \| issue = 3 \| pages = 129-36 \| month = \| year = 1991 \| doi = \| PMID = 1915756 }}</ref>		*The thickness of the sections can make a difference in the interpretation.<ref name=pmid1915756>{{Cite journal \| last1 = Otto \| first1 = MJ. \| last2 = Löw \| first2 = O. \| last3 = Schneider \| first3 = A. \| title = The nominal section thickness--importance of their correction for morphometry. \| journal = Exp Pathol \| volume = 42 \| issue = 3 \| pages = 129-36 \| month = \| year = 1991 \| doi = \| PMID = 1915756 }}</ref>
	**Routine sections are cut at 3-4 micrometres.		**Routine sections are cut at 3-4 micrometres.
	***3 micrometres is considered ideal.<ref>LE. 20 January 2015.</ref>		***3 micrometres is considered ideal for GI biopsies and prostate biopsies.<ref>LE. 20 January 2015.</ref>

	===Staining===		===Staining===

Michael: /* Cutting */

2015-01-20T17:48:27Z

Cutting

← Older revision		Revision as of 17:48, 20 January 2015
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	*Tissue is cut from the block.		*Tissue is cut from the block.
	**Pieces of tissue sent to the pathologist are tracked as "levels".		**Pieces of tissue sent to the pathologist are tracked as "levels".
	**Who cut the tissue is tracked.		**Who cut the tissue is tracked.

			Note:
			*The thickness of the sections can make a difference in the interpretation.<ref name=pmid1915756>{{Cite journal \| last1 = Otto \| first1 = MJ. \| last2 = Löw \| first2 = O. \| last3 = Schneider \| first3 = A. \| title = The nominal section thickness--importance of their correction for morphometry. \| journal = Exp Pathol \| volume = 42 \| issue = 3 \| pages = 129-36 \| month = \| year = 1991 \| doi = \| PMID = 1915756 }}</ref>
			**Routine sections are cut at 3-4 micrometres.
			***3 micrometres is considered ideal.<ref>LE. 20 January 2015.</ref>

	===Staining===		===Staining===

Michael: /* Prior to arrival to the lab */

2015-01-19T18:46:07Z

Prior to arrival to the lab

← Older revision		Revision as of 18:46, 19 January 2015
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	*Specimen descriptors:		*Specimen descriptors:
	**How many parts, i.e. how many containers.		**How many parts, i.e. how many containers.
	***One container = at least on diagnosis.		***One container = at least one [[diagnosis]].
	***Anatomical site of the parts, e.g. kidney biopsy.		***Anatomical site of the parts, e.g. kidney biopsy.

	The above information is all contained on the requisition.		The above information is all contained on the requisition.

	===Accessioning===		===Accessioning===

Michael: /* See also */

2015-01-19T18:43:06Z

← Older revision		Revision as of 18:43, 19 January 2015
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	*[[Fixation]].		*[[Fixation]].
	*[[Laboratory information system]].		*[[Laboratory information system]].

			==External links==
			*[http://protocolsonline.com/histology/sample-preparation/paraffin-processing-of-tissue/ Sample preparation (protocolsonline.com)].

	[[Category:Quality]]		[[Category:Quality]]