View source for H3F3A

[[File:K27M_mutant_diffuse_glioma_of_the_midline.jpg|thumb|right|250px|K27M staining in a diffuse glioma of the midline.]]
'''H3F3A (K27M)''', often abbreviated '''K27M''' or '''H3.3 K27M''' is a mutation-specific antibody detecting the K27M mutation in tumor nuclei.<ref>{{Cite journal  | last1 = Venneti | first1 = S. | last2 = Santi | first2 = M. | last3 = Felicella | first3 = MM. | last4 = Yarilin | first4 = D. | last5 = Phillips | first5 = JJ. | last6 = Sullivan | first6 = LM. | last7 = Martinez | first7 = D. | last8 = Perry | first8 = A. | last9 = Lewis | first9 = PW. | title = A sensitive and specific histopathologic prognostic marker for H3F3A K27M mutant pediatric glioblastomas. | journal = Acta Neuropathol | volume = 128 | issue = 5 | pages = 743-53 | month = Nov | year = 2014 | doi = 10.1007/s00401-014-1338-3 | PMID = 25200322 }}

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==Positive staining==
*Nuclear
**Approx 30% of pediatric glioma.
**Approx 80% of diffuse intrinsic pontine glioma (DIPG).

Note:
*The antibody does not detect other H3F3A mutations (ie. G34V or G34R).

==Normal tissues==
*Absent

==See also==
*[[Immunohistochemistry]].

==References==
{{Reflist|1}}

[[Category:Immunohistochemistry]]
[[Category:Neuropathology]]